FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003490723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469775
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211130.8548551993602657No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT179060.7250053142492737No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA124180.5027988379508255No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC112730.4564383395248555No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC107980.4372058183437762No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC104300.4223056756182243No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC94800.3838406332560658No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG91050.36865706390258224No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT87750.3552955228715166No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC85940.34796692006356855No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT85490.3461448917411505No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT85370.34565901752183903No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG73700.298407749693798No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA66930.2709963458209756No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG64070.2594163435940521No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT63000.25508396513852477No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC62580.2533834053709346No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC54660.2213157068963772No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC53260.2156471743377433No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC52240.21151724347359574No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCT49470.2003016469111559No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA48390.19592877893735258No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC47550.19252765940217229No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA47360.1917583585549291No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC46670.18896458179388811No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT46550.18847870757457663No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT44330.17949003451731432No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC42790.17325464870281707No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG42410.17171604700833074No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG41590.16839590650970232No Hit
TGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCA41570.16831492747315038No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT39990.1619175835855493No Hit
AATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGC39270.15900233826968044No Hit
CTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACA38930.15762569464829793No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT37670.15252401534552743No Hit
GATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTC37130.15033758135862577No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC35670.14442611169033617No Hit
ATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCA35370.14321142614205748No Hit
GTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAG34120.13815023635756293No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC34010.1377048516565274No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC33460.13547792815134982No Hit
CACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGA33420.135315970078246No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT33120.1341012845299673No Hit
TGATAATCTCAAGACACCTCCCTTAGCTACTCAGGAGGCTGAGGCAGAAA32880.13312953609134437No Hit
TAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAG32170.13025478029375145No Hit
CACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCA31780.12867568908098917No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC31430.12725855594133068No Hit
CCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGC31410.1271775769047788No Hit
CCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGG31200.1263272970209837No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAG30780.12462673725339353No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT30630.12401939447925418No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA29870.1209421910902815No Hit
CAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTT29560.11968701602372686No Hit
AGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC29210.11826988288406838No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC29030.11754107155510117No Hit
ACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACT28720.11628589648854652No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT28720.11628589648854652No Hit
AGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACA28270.11446386816612848No Hit
CCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCC28180.11409946250164489No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC28170.11405897298336894No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT28030.11349211972750553No Hit
AAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAG27770.11243939225233068No Hit
CTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGT27670.11203449706957112No Hit
GGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGG27420.11102225911267222No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA27340.11069834296646457No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT26970.10920023079025416No Hit
GCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAG26390.1068518387302487No Hit
CAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGG26330.10660890162059297No Hit
CGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGA26220.10616351691955744No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG25970.10515127896265854No Hit
GATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA25970.10515127896265854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)