FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003490740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169148
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG78820.3633684746269042No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT76780.3539638604650305No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA63140.29108202851995346No Hit
GTGAAGGGTGTGGTTAGCCTGCTGCTGGTGGCTGAGGTGGTGTGGGCCAC47320.21815016771561924No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC47190.21755085406804883No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG46750.21552240787627214No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT46340.2136322648339348No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC40680.18753907063971662No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT37780.17416976619391578No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA36860.16992846961111No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT36260.16716240662232362No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG36170.16674749717400564No Hit
GTGGTGAAGGGTGTGGTTAGCCTGCTGCTGGTGGCTGAGGTGGTGTGGGC34190.1576194893110106No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA32570.15015111924128738No Hit
GGTGGCTGTAGTGCTCGGCTCTGTGAGGATCCAGGTGGTCCCTGGGGTGG32330.1490446940457728No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG31190.1437891743670787No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT30780.14189903132474133No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC30780.14189903132474133No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT28810.1328171245115594No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT27750.1279304132313701No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC27650.12746940273323906No Hit
GTGGTGAATGGTGTAGTTAGCCTGCTGCTGGTGGCTGAGGTAGTGTGGGC27580.12714669538454731No Hit
GGTGAATGGTGTAGTTAGCCTGCTGCTGGTGGCTGAGGTAGTGTGGGCCA26160.1206003463110862No Hit
GGGGAGTGTGTGGTGAAGGGTGTGGTTAGCCTGCTGCTGGTGGCTGAGGT24260.11184114684659599No Hit
GTGTGGTTAGCCTGCTGCTGGTGGCTGAGGTGGTGTGGGCCACGGGTGTC23490.10829136601098681No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC23420.10796865866229506No Hit
GTGTGGTTAGCCTGCTGCTGGTGGCTGAGGTGGTGTGGGCCACAGGGGTG22660.10446497887649897No Hit
GTGGCTTTGGAGCTGGTGACTGTGGGTGTCGTGGCCGTGGTGCTCACATG22400.1032663515813582No Hit
GGTGAAGGGTGTGGTTAGCCTGCTGCTGGTGGCTGAGGTGGTGTGGGCCA22090.10183721903715191No Hit
GTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTTGGGGTCACCGTAGTGGT22020.10151451168846017No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT21910.101007400140516No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG21900.1009612990907029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)