FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538042
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA55290.35948303102255985No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA36660.23835499940833862No Hit
GGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGAC33640.21871964484714979No Hit
GTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACG31040.20181503496003358No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG30730.19979948531964664No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT29910.19446803143217156No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG29090.18913657754469643No Hit
CTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAG27840.18100936125281364No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG27600.17944893572477214No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA26240.17060652439920365No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG26230.1705415066688686No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA24290.1579280669838665No Hit
GTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTAAGACG23590.15337682586041215No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG21940.14264890035512684No Hit
GTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCAAAGA21570.14024324433272953No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT21290.1384227478833478No Hit
GGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTAAGAC21060.13692734008564134No Hit
GGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCA20710.13465171952391417No Hit
CTCTAAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCTGGATGTTGTAG20170.13114076208582082No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC19960.1297753897487845No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG19940.12964535428811436No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT18640.12119304934455626No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG18300.11898244651316414No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG17890.11631671956942657No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG17640.11469127631105003No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT17630.11462625858071496No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG16520.10740929051352305No Hit
CTGGTCAGGAGGGATGCCTTCCTTGTCTTGGATCTTTGCCTTGACATTCT16390.10656406001916723No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC15850.10305310258107386No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT15500.10077748201934666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)