FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001683
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGAAGTTGTGGAGCTGGCAGGGAAGTGTGTTGTTGGAGTCGAGCCTG85470.4269906873366062No Hit
CCCGAAGTTGTGGAGCTGGCAGGGAAGTGTGTTGTTGGAGTCGAGCCTGG68020.33981404647988717No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC39480.19723402756580335No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA36940.18454470563021216No Hit
GCCTGAGGTTGTGGAGCGGGCAGGCGAGGGTGTTGTTGCAGTTGCAACTG34880.17425336579268547No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA34530.17250483717951345No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT32220.16096454833257814No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT30400.15187219954408365No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG27130.1355359465010194No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG26060.13019044474075067No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG25940.12959094921623454No Hit
GTGGGACTGCCGGGTAAAGTTGTTGTTTCGGTTGAGCTTGGACTGAGGCG25290.12634368179177222No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA25230.12604393402951417No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA25200.12589406014838514No Hit
GTGGGAAGTTGTAGGTTCCTGACTGAGGCCTGAGGTGGTGGTGCTGTCAG25150.1256442703465034No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG25150.1256442703465034No Hit
CTGGCGTGGTGGTGCTGGCAGGTGACACTGTTGAGTGAGTAGAACCTGGT25100.1253944805446217No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG24030.12004897878435297No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG23970.1197492310220949No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG21870.1092580593430628No Hit
GTCCGAAGTTGTGGAGCTGGCAGGGAAGTGTGTTGTTGGAGTCGAGCCTG21310.10646041356198759No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT21020.10501163271107364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)