FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003490762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726383
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA99871.3748945115730957No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG56460.7772758999040451No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG54400.7489162053627356No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT54320.7478148580019081No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA35300.48596952296515755No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG32690.4500380653181586No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG31230.4299384759830558No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC30840.42456940759902145No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT29380.4044698182639186No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG29300.403368470903091No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG28080.3865729236504709No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT27150.3737697605808506No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA26630.3666110027354715No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC25920.356836544908127No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT25760.35463385018647187No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT25700.3538078396658512No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG25080.3452723976194377No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT24120.33205622928950707No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT23810.32778850826630024No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT22630.31154363469409385No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC21970.3024575189672666No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG21600.29736378742343916No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT21330.2936467400806462No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG21260.29268306113992204No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA21040.28965435589764627No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA21010.2892413506373359No Hit
CTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGT20870.2873139927558877No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG20570.2831839401527844No Hit
AGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGAT20020.275612177047095No Hit
TGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAG19790.27244580338471575No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA19270.2652870455393367No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG19050.2622583402970609No Hit
CTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTA18920.2604686508357162No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC18470.25427357193106115No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA17400.23954305097999262No Hit
CTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTG17380.23926771413978576No Hit
CCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTG17240.23734035625833755No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC17160.23623900889751No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT16890.232521961554717No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA16800.231282945773786No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA15850.21820444586395882No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG15360.21145869327889008No Hit
GGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAG15270.21021967749795906No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC15010.20664029857526953No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC14840.20429993543351097No Hit
GGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAA14720.20264791439226965No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA14530.20003221441030422No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC14420.19851786178916633No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC14160.19493848286647678No Hit
GTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGT14040.19328646182523546No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT13170.1813093092762358No Hit
GGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGC12960.1784182724540635No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC12860.17704158825302904No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG12830.17662858299271872No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC12310.16946982514733963No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC12280.1690568198870293No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC12120.16685412516537418No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT11980.164926767283926No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC11850.1631370778225812No Hit
GTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTG11740.16162272520144333No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC11510.1584563515390641No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC11440.15749267259834No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC11400.15694199891792623No Hit
TGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCG11320.15584065155709867No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTT11220.1544639673560642No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA11120.15308728315502979No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT10980.15115992527358157No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC10800.14868189371171958No Hit
CAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGG10610.14606619372975416No Hit
GCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATT10510.1446895095287197No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG10440.1437258305879956No Hit
CAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGC9990.1375307516833406No Hit
GTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAA9620.13243702013951317No Hit
AGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCA9530.13119800435858217No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC9420.1296836517374443No Hit
CGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGA9410.12954598331734085No Hit
CGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTG9360.1288576412168236No Hit
CCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAG9320.12830696753640985No Hit
CGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCC9270.12761862543589264No Hit
CACTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACG9100.12527826229413408No Hit
CCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCC9040.12445225177351342No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA8970.12348857283278933No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC8810.12128587811113421No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT8800.12114820969103077No Hit
CGCTGAGGGTGGAAGGGGAGTGAGCACACACTCGGGAGGTGTCTTGAGAT8760.12059753601061698No Hit
CGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTT8730.12018453075030666No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG8640.11894551496937567No Hit
CAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTGC8500.11701815708792744No Hit
CACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGA8460.11646748340751367No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG8440.11619214656730678No Hit
CTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAAGATCT8350.11495313078637578No Hit
CCTTGACATTCTCGATGGTGTCACTGGGCTCCACCTCGAGGGTGATGGTC8340.11481546236627234No Hit
CTTGACATTCTCGATGGTGTCACTGGGCTCCACCTCGAGGGTGATGGTCT8320.11454012552606545No Hit
GCCCGCTCTGTTTTCCCTGAACCAGGAGTCTCCAGATGTCAACACAGAAG8280.11398945184565167No Hit
CCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC8140.11206209396420347No Hit
CAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGC8000.11013473608275524No Hit
AAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTG7920.1090333887219277No Hit
GGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCA7860.10820737820130702No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG7840.10793204136110013No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG7840.10793204136110013No Hit
AGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAA7820.10765670452089326No Hit
CCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCA7670.10559167821934158No Hit
CTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCA7660.10545400979923815No Hit
CTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAG7300.10049794667551415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)