FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094373
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC41060.375192004919712No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC38940.3558201819672086No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC27260.2490924026817182No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT26670.24370118780342717No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC23440.21418657075786776No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC22790.20824709673941152No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG22070.20166798705742922No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC21950.20057146877709886No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT21540.1968250313193034No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA21240.19408373561847744No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA20910.19106831034756888No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC20840.19042867468404284No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC20800.19006316859059938No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT20370.18613397808608215No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT19150.17498604223605663No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT19110.17462053614261316No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC18630.17023446302129167No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC18170.16603114294669186No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC17980.1642949890028354No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG16620.15186778182575775No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA16570.15141089920895343No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT16470.15049713397534478No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC16220.14821272089132315No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG15340.14017158683556705No Hit
ATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTAT14880.13596826676096724No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT14820.13542000762080203No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC14750.134780371957276No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC14530.13277008844333696No Hit
CAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCATGCC14490.1324045823498935No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG14370.1313080640695631No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG14060.1284753918453763No Hit
CTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATTGGAGGAACAGTACC13920.12719612051832418No Hit
AGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGG13740.12555134309782862No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT13710.12527721352774604No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT13690.1250944604810243No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC13640.12463757786421996No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT13560.12390656567733305No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC12980.11860672732240288No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT12930.11814984470559856No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT12260.11202761764042059No Hit
CTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACA12240.11184486459369886No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC12200.1114793585002554No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC12170.1112052289301728No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG11810.10791567408918167No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT11460.10471749577155137No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA11400.10416923663138619No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT11320.10343822444449927No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA11320.10343822444449927No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG11170.1020675765940863No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG11150.10188482354736457No Hit
GGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGC11140.10179344702400371No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC11130.10170207050064284No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT11010.10060555222031245No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA10960.10014866960350813No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA10960.10014866960350813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)