FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440364
Sequences flagged as poor quality0
Sequence length101
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC79910.5547903168921189No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT75340.5230622259373324No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC56150.3898320146851768No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC51810.35970074231235993No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA49330.342482872385036No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC40580.28173433937532455No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT40310.27985981321388204No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC39440.273819673360345No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT38690.2686126562452269No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA35700.24785401467962268No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC34460.2392450797159607No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT33340.23146926749071764No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG29620.2056424625997317No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC29000.20133799511790076No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG27660.19203479120555636No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT27490.19085453399279628No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT26360.18300929487268497No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC25680.17828826602164452No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT25540.17731628949348913No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG25450.17669144743967496No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT24980.1734283833808676No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC24880.1727341144321852No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC24540.170373600006665No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA24540.170373600006665No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC23380.16232008020194894No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG23120.16051498093537467No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC21910.15211432665631744No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG21660.1503786542846114No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA20800.14440794132594262No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA19710.13684040978530426No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG19590.13600728704688536No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG19410.13475760293925704No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT19340.13427161467517934No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC19290.1339244802008381No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC19210.1333690650418922No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT19140.1328830767778145No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC18700.12982829340361185No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT18660.1295505858241389No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18610.1292034513497977No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA17930.12448242249875727No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC17630.12239961565271001No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA17530.12170534670402759No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG17020.11816457506574726No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC16710.11601234132483178No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG16640.1155263530607541No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA16290.11309641174036564No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT16010.11115245868405486No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG16000.11108303178918662No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC15510.10768111394064278No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG15460.10733397946630158No Hit
AGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC15400.10691741809709211No Hit
CCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCG15290.10615372225354146No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC15170.10532059951512257No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT15120.10497346504078137No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC15100.10483461125104487No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT14960.1038626347228895No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT14750.1024046699306564No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT14690.10198810856144698No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC14620.10150212029736928No Hit
CTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCC14510.10073842445381861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)