FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003490785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489919
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC86040.34555340956874503No Hit
CTTTGCCGGAAAGCAGCTGGAAGATGGGCGCACCCTGTCTGACTACAACA50380.2023358992802577No Hit
GGAAGGCATTCCTCCTGACCAGCAGAGGTTGATCTTTGCCGGAAAGCAGC48120.19325929879646686No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT47670.19145201108951737No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC47090.1891226180450047No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC42100.16908180547238685No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC41330.16598933539605104No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC39590.15900115626251296No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC37560.1508482806067185No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC36600.1469927334985596No Hit
CTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACA35000.14056682165162804No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC34950.14036601190641143No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT34360.1379964569128554No Hit
AGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTG34290.13771532326955213No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC33740.13550641607216943No Hit
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT33410.13418107175373978No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA33220.13341799472191665No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG32570.1308074680341007No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA31900.12811661744819813No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC31480.12642981558837857No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA30250.12148989585604993No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC30180.12120876221274669No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT29680.11920066476058055No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG29310.11771467264597765No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC28580.1147828503658151No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG28580.1147828503658151No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT28160.11309604850599558No Hit
CACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCA27720.11132892274808939No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA27510.11048552181817962No Hit
AGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAA27360.1098830925825298No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT27060.10867823411123012No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT26570.10671029860810732No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC26520.10650948886289072No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC26100.1048226870030712No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT26020.1045013914107246No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG26010.10446122946168128No Hit
CCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCC25830.10373831437890148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)