FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863467
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA105120.5641098017834498No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG83280.44690890689236784No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA66600.3573983333217063No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA60010.32203414388341733No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT56260.30191036385404196No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG54100.29031906655712175No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG51380.27572261810914817No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA45740.24545645294496762No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG45220.24266595544756095No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG40620.2179807852781938No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT40480.21722949749043047No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC38640.20735542942268365No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT38170.20483324899233527No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT37380.20059383933281352No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT36720.19705205404764342No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG36180.1941542297234134No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG33200.17816253252673644No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA32810.17606965940368144No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG32770.17585500575003474No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG32750.17574767892321141No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC31740.17032767416863298No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG31520.1691470790735763No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT31510.16909341566016461No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC31180.1673225230175796No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA29440.15798508908394943No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG28970.15546290865360107No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT28350.15213577702207765No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC28320.15197478678184267No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC28160.15111617216725598No Hit
GTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGT27880.1496135965917293No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG27800.14918428928443594No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT27360.14682309909432256No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC26850.14408626501032754No Hit
CTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTG26520.1423153723677425No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC25760.13823695294845575No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC25740.1381296261216324No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA25630.13753932857410409No Hit
CTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGT25590.1373246749204574No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC24980.1340512067023457No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG24630.13217298723293733No Hit
TGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAG24090.12927516290870725No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT23870.12809456781365058No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC23690.12712862637224057No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC23500.12610902151741887No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT23330.1251967434894205No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC23140.12417713863459884No Hit
CTTGAGATTATCATCGGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG22700.12181594844448546No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA22360.11999139238848876No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC22100.11859614363978542No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG21220.11387376325955867No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC20780.11151257306944529No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA20290.10888306581227358No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA19980.10721949999651188No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG19780.10614623172827853No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG19660.10550227076733852No Hit
GTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAA19430.10426801225887015No Hit
GGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAA18840.10110187086758177No Hit
AGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGAT18810.10094088062734677No Hit
CAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGC18760.10067256356028843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)