FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149779
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT35840.3117120768425932No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC25160.21882466108704368No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA25130.21856374137986517No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC24370.21195377546467625No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC22990.1999514689344648No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC22400.19482004802662076No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT21950.19090625241894313No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT21850.19003652006168142No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC20510.17838210647437464No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT18690.1625529775722117No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC18320.15933496785034343No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT18040.15689971725001065No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT17930.15594301165702276No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT17510.15229013575652364No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA16610.14446254454116833No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA16460.1431579460052758No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG16330.14202729394083558No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC15840.13776560539025326No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC15150.13176445212514754No Hit
CTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC14450.12567632562431563No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC14430.12550237915286327No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG14100.12263226237389968No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC14080.12245831590244735No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC13700.11915333294485288No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC13610.11837057382331735No Hit
GTGGAAGAAGACCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCT13510.11750084146605565No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG13090.11384796556555651No Hit
CAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACA13050.11350007262265183No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT12880.11202152761530695No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC12820.11149968820094991No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC12750.11089087555086674No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC12730.11071692907941438No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA12620.10976022348642653No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC12390.1077598390647246No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC12180.10593340111447504No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC12100.10523761522866569No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC11860.10315025757123761No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG11790.10254144492115441No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC11650.10132381962098802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)