FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6289191
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1562092.4837693751072276No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC504740.8025515523379717No Hit
CGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGA428250.680930186410303No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG321510.5112104243614163No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG272410.43313996983077796No Hit
GTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCA254470.4046148383790539No Hit
GGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAA238930.3799057780245504No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG230040.3657704146685957No Hit
GCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTA221360.3519689575336478No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA202300.3216629929032208No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT199720.31756071647370865No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA188350.299482079650626No Hit
GGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGC187240.2977171467681614No Hit
CGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACAC161760.2572031919526693No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGA161390.25661488099184776No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG148360.23589679499318753No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT146420.23281213752293417No Hit
GAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCT144720.23010908716240291No Hit
CATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAAC142860.22715163206205696No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA134500.21385898440673848No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA129840.20644944635963514No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG129270.20554312947404524No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC128350.20408030222011067No Hit
CAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAGAT127880.20333298829690494No Hit
GGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAA119990.1907876545647922No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA117690.18713058642995578No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG115980.1844116357731861No Hit
GGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAA115850.18420493192208665No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTG110000.1749032586226114No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA99840.15874855764437748No Hit
GACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCC99430.15809664549860228No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG98280.15626811143118408No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA97800.15550489721173993No Hit
GAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCG97230.15459858032615006No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG93950.14938328315994856No Hit
CGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAAC87160.13858698201406192No Hit
GACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGA87160.13858698201406192No Hit
GGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGG87080.13845977964415454No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC86920.13820537490433984No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG86560.13763296423975677No Hit
GGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCA84330.13408719817858927No Hit
ATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTG82610.13135234722558115No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC82370.1309707401158591No Hit
GGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCC81110.12896730278981827No Hit
GAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAAT81070.12890370160486458No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG80990.12877649923495724No Hit
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG78190.12432441628819986No Hit
GCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTG75250.11964972919410462No Hit
CGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACAC74370.11825050312512372No Hit
GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG74190.11796429779283217No Hit
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG74070.11777349423797115No Hit
AGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCG73400.11670817438999706No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG72830.11580185750440716No Hit
CAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCA72050.11456163439781046No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA70030.11134977455764979No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT69900.11114307070655034No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC69240.11009365115481466No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA68010.1081379147174891No Hit
GAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTG67240.10691359190713082No Hit
AGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGAT64430.10244560866413503No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC64080.10188909829579033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)