FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003490809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091818
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT38500.35262287304294304No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC35440.3245962239127767No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT30030.2750458409734956No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC27500.2518734807449593No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA24700.22622818088729074No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC24560.22494591589440732No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC22760.20845965170019182No Hit
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC20460.18739386967424973No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT19200.17585348473829887No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA18410.16861784656417095No Hit
CCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCC18370.16825148513763283No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG17610.1612906180334085No Hit
CACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG17160.15716905198485462No Hit
CACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACGCCA16950.15524565449552946No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA16590.15194840165668638No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC16580.15185681130005182No Hit
CCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGC16220.14855955846120875No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT14850.13601167960227806No Hit
CATCTGCTCAGCTTCCCAGTCCCAGCCGACACCCAGCCCAGACACTTGGC14850.13601167960227806No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC14670.1343630531828565No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG14500.13280601712006948No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC14360.13152375212718603No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC14120.1293255835679573No Hit
GTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAACTGTG13940.12767695714853575No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG13810.12648628251228686No Hit
CATGAGTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAA13710.12557037894594153No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT13430.12300584896017468No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT13310.12190676468056032No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG13260.12144881289738767No Hit
GTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCC13150.12044131897440782No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC12660.11595339149931581No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT12540.11485430721970145No Hit
CACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCA12100.11082433152778211No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT12010.11000001831807132No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA11950.10945047617826413No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT11940.10935888582162961No Hit
CCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGCTCAGGACATG11680.10697753654913182No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT11590.10615322333942104No Hit
CCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCC11490.10523731977307572No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC11430.10468777763326856No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC11420.10459618727663401No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT11270.10322233192711606No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG11230.10285597050057793No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC11000.10074939229798373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)