FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003490811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057451
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT32350.3059243407023115No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA30070.2843630579572954No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT29280.2768922626202065No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC27340.25854625888102617No Hit
CAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACA27260.2577897226443589No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT26740.2528722371060219No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC22860.21618022962766123No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC22540.21315408468099234No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA21710.20530502122556982No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC18400.1740033344334631No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA17480.16530316771178996No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT17110.161804187617204No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC16530.1563192999013666No Hit
CTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC16480.15584646475344957No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC16250.15367142307303128No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC14250.1347580171563505No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT13890.13135360409134797No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC13630.12889486132217945No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT13350.12624698449384417No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT13150.12435564390217609No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC13100.12388280875425906No Hit
GTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGC13000.12293713845842502No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT12970.12265343736967481No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC12640.1195327253934225No Hit
CCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCC12410.11735768371300419No Hit
CTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT12370.1169794155946706No Hit
CAACCTCTGGTGCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA11550.10922491916883148No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC11280.10667160937007955No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG11050.10449656768966127No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA10850.1026052270979932No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT10810.10222695897965958No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG10780.10194325789090937No Hit
GGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC10740.10156498977257575No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG10730.10147042274299234No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC10610.10033561838799149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)