FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988084
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA113080.37843648304398403No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG97380.3258944527663881No Hit
GCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACC91880.3074880090385678No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG84470.28268950939799553No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT82440.2758958583493637No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC78770.26361374044370905No Hit
GTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCC78190.26167269728695713No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGT73080.24457143775074597No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA69230.2316869271412718No Hit
GCTGGTTGTAGGGGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGC68080.22783830708909122No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT60620.2028724761419023No Hit
GGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGG59280.19838799712457883No Hit
GCTGGTTGTAAGGGGAGAGATTGTGCTGGTTGTAGAAGCAGAGGTTGTGC58860.19698241414899983No Hit
GCTAGTTGTAGGAGCAGAGGTTGTGCTCGTGGTGGGAACAGGGCTGAGAG58240.19490750594695463No Hit
GTGCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGT57950.19393698436857867No Hit
GCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGC56740.18988756674845822No Hit
GTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGG56390.1887162476021424No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA54300.18172179898557067No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA52330.1751289455048787No Hit
GGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAG51460.1722173807697508No Hit
GTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT51340.17181578563387107No Hit
GCTGGTTATAGGAGCAGAGGTTGTGCTGGTTGTAAGGGGAGAGATTGTGC47350.15846274736587057No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC46420.15535038506280277No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG46070.15417906591648695No Hit
GGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGG44650.149426856808577No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG44270.14815513887829124No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC43050.14407225499684748No Hit
GGCAGAGGTTGTGCTGCTTATAGGGGCAGAGGTTGTGCTGGTTGTAGGAG42590.14253280697597526No Hit
AGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAG42250.1413949540909827No Hit
GTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCT41600.1392196471049676No Hit
GGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTG41100.13754633403880212No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG41070.13744593525483217No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT40550.1357056896660201No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC40450.135371027052787No Hit
GCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGC39410.13189053587516283No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT38800.12984909393444094No Hit
GGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGTGGGAA38770.12974869515047102No Hit
GCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTAGTTGTAGGAGCAGAGG38480.12877817357209503No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTACTTCCAGGACCAGAGGTTGT38360.12837657843621533No Hit
GGCAGAGGTTGTGCTGGTTGTTGGGGCCGAGATTGTGCTGGTTGTAGGGG37970.12707139424460626No Hit
AGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAG36900.12349050428301213No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC36600.12248651644331285No Hit
GCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAAC35920.1202108106733278No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG35790.11977574927612476No Hit
GCTGGTTGTAGGAACAGAGATTGTGCTGGTTGTAGTAGCTGAGGTTGTGC34410.11515740521350806No Hit
GCTGGTTATAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG34210.11448807998704186No Hit
GTAGGGGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGT34200.11445461372571857No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA34070.11401955232851553No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTGGTTCCAGGACCAGAGGTTTT33260.11130878516132744No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG32810.10980280340177853No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC31990.10705856997326715No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC31820.10648964353077088No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG31200.1044147353287257No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC30830.10317648365976324No Hit
GCTGGTCTTTGAAACAGGGGTTGTGCTGGTGGTGGGAACGGGGCTGGGAG30380.1016705019002143No Hit
GTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGG30360.1016035693775677No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC30110.10076691284448497No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG30060.10059958153786841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)