FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209502
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC113280.5126947158228415No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA111810.5060416329109456No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT92990.4208640680117058No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG78850.35686774666870635No Hit
GTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCC65000.29418393828111494No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA53660.24286015581791734No Hit
GTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT48580.2198685495645625No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG47450.2147542749452139No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA47400.2145279796080746No Hit
GCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACC46360.20982103659557674No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGT46340.209730518460721No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA46050.20841800550531298No Hit
GCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGC44040.19932093295231235No Hit
GGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGTGGGAA40380.18275611427371416No Hit
GTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCT40010.1810815287788832No Hit
GTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGG39770.17999531116061446No Hit
CTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGT39360.17813968939607205No Hit
GCTGGTTGTAGGGGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGC38440.17397585519270858No Hit
GCTAGTTGTAGGAGCAGAGGTTGTGCTCGTGGTGGGAACAGGGCTGAGAG36450.16496930077456368No Hit
GTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGG35530.1608054665712002No Hit
CTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCT35080.15876880853694633No Hit
GCTGGTTGTAAGGGGAGAGATTGTGCTGGTTGTAGAAGCAGAGGTTGTGC32950.14912862717481135No Hit
AGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAG32580.14745404167998039No Hit
GGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGG32010.14487427483659213No Hit
GGCAGAGTTTGTGCTGGTTGTAGGTGCAGAGGTTATGCTGGTGGTGGGAA31830.14405961162289058No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT31640.14319968934176117No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA31480.14247554426291537No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTCATGCTGGTTAT30050.13600349762073083No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT29730.13455520746303917No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT29380.13297114010306393No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTACTTCCAGGAC29190.13211121782193452No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGTTGT28920.1308892230013822No Hit
GGCAGAGGTTGTGCTGCTTATAGGGGCAGAGGTTGTGCTGGTTGTAGGAG28740.13007455978768068No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC28620.12953145097854632No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG28570.12930515564140696No Hit
GCTGGTTATAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG28440.12871678776484474No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG27860.12609176185402865No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC27760.12563917117975No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT27630.12505080330318777No Hit
GGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAG27340.12373829034777974No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTAGTTGT27220.12319518153864537No Hit
CAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCA27200.12310466340378964No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA27190.12305940433636176No Hit
GGCAGAGATTGTGCTGGTTGTAGGGGCTGAGGTTCTTCTGGGTGTAGGAG27030.12233525925751595No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTTCTGGTTGTAGGAG26370.11934816080727693No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT26340.11921238360499335No Hit
GGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGG26200.11857875666100326No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC25810.11681365303131655No Hit
GCTGGTTATAGGAGCAGAGGTTGTGCTGGTTGTAAGGGGAGAGATTGTGC25720.11640632142446579No Hit
GTTGTGCTTGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT25700.11631580328961007No Hit
AGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAG25090.11355500017651037No Hit
GTTCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGG24120.10916487063600756No Hit
GCTGGTTGTAGGAACAGAGATTGTGCTGGTTGTAGTAGCTGAGGTTGTGC23920.10825968928745029No Hit
GTGCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGT23780.1076260623434602No Hit
GTGGGAACAGGGCTGAGAGAAGTTCCAGGACCAGAGGTTGTGCTGGCTGT23670.10712821260175369No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC23440.10608725405091284No Hit
GCTGGCTGTAGAGGCAGGGGTTGTTCTGGTTGTAGGAGCAGAGGTTGTGC23060.10436740948865401No Hit
ACCAGAGGTTGTGCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAG23010.1041411141515147No Hit
GCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTAGTTGTAGGAGCAGAGG22800.10319067373552955No Hit
GGCAGAGGTTGTGCTGGTTGTTGGGGCCGAGATTGTGCTGGTTGTAGGGG22220.10056564782471343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)