FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209502
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT120390.5448739127640527No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA97510.44132116648910025No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC79820.36125787620920913No Hit
CAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACA62920.28477005225611923No Hit
CAACCTCTGGTCCTGGAACTTCTCTCAGCCCTGTTCCCACCACGAGCACA57660.26096378278906285No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC50810.22996132160097615No Hit
CTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC49260.22294616614965726No Hit
CTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT43180.19542865315351604No Hit
CAATCTCTCCCCTTACAACCAGCACAACCTCTGCTCCTATAACCAGCATG38220.17298015570929556No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA37710.17067194327047452No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC37320.16890683964078781No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC35970.16279686553802622No Hit
CTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAGCCAGCACAACCT34970.15827095879523984No Hit
CTCTGCTTCTACAACCAGCACAACCTCTGGTCCTGGAACTACTCCCAGCC34570.1564605960981253No Hit
GTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGC33390.15112002614163733No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT33350.1509389898719259No Hit
CACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTCTA32110.14532686551087076No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC31110.14080095876808438No Hit
CCTGTTCCTACCACCAGCACAACCTCTTCTCCTACAACCAGCACAACCTC31070.14061992249837293No Hit
CAACCTCTTCTCCTACAACCAGCACAACCTCTGCTCCTACAACCAGCACA30500.1380401556549847No Hit
CCAGCACAACCTCTGCCTCTACAGCCAGCACAACCTCTGGTCCTGGAACT28710.12993878258539707No Hit
CCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCAC28170.12749479294429245No Hit
CTCTGTTCCTACCACCAGCACAACTTCTGCTTCTACAACCAGCACAACCT27090.12260681366208312No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC26940.12192792765066518No Hit
CCCAGCTTCACTTCTTTGATCACCACCACGGAGACCACCTCACACAGTAC26740.12102274630210788No Hit
ATCTGCTCCTACAAGCAGCATGACCTCTGGTCCTGGAACTACTCCCAGCC26600.12038911935811782No Hit
CCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTC26570.1202533421558342No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGA26180.11848823852614752No Hit
CCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCC26120.11821668412158033No Hit
CACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAG25640.11604424888504287No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACAAGCACA25550.11563691727819209No Hit
CCAGCACAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACC25530.11554639914333638No Hit
CCTACAACCAGCACAACCTCTGCCTCTACAGCCAGCACAACCTCTGGTCC25410.11500329033420202No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC25210.11409810898564472No Hit
CCCCTGCCTCTACAGCCAGCACAACCTCTGGTCCTGGAACTACTCCCAGC24960.11296663229994815No Hit
CCTCAAGCAGTACTCCCAGCTTCACTTCTTCGATCACCACCACCGACTCG24710.11183515561425153No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCTACCACCAGCACAATCT24680.11169937841196795No Hit
TGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTA24300.10997953384970913No Hit
CCAGCACAACCTCTGCTCCTATAACCAGCATGACCTCTGGTCCTGGAACT24250.10975323851256981No Hit
GCACAACTTCGGCTCCTACAACCAGCACAACTTCTGGTCCTGGAACTACT23820.10780709861317167No Hit
CTCTCCCCTTACAACCAGCACAACCTCTGCTCCTATAACCAGCATGACCT23060.10436740948865401No Hit
GCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAGCCAGC23060.10436740948865401No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT22490.10178764264526577No Hit
CTCTGCTTCTACAACCAGCACAATCTCTCCCCTTACAACCAGCACAACCT22320.1010182384989921No Hit
ACCAGCACAACCTCTGCTCCTATAACCAGCATGACCTCTGGTCCTGGAAC22170.10033935248757411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)