FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240819
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC29700.23935803690949284No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC28200.22726924716658914No Hit
CCCAGCCCAGACACTTGGCCAACCTCACATGCATCAACAGCAGGATCTGA24300.1958383938350396No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC22890.18447493147671015No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT22190.1788334962633551No Hit
CCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCC19650.15836314563203818No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT19170.154494732914309No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC18780.15135164758115408No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC18180.14651613168399258No Hit
CCACAGGTCACGCCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC17030.13724805954776642No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC16840.13571681284699863No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA16650.13418556614623084No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA16350.1317678081976501No Hit
CCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGC16210.1306395211549791No Hit
GTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAACTGTG15140.1220161844717078No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA14760.1189536910701722No Hit
GGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAG14750.11887309913855285No Hit
CATCTGCTCAGCTTCCCAGTCCCAGCCGACACCCAGCCCAGACACTTGGC13730.11065272211337833No Hit
CAATGATGCCAATGTGGTTTGCAGGCAGCTGGGCTGTGGCTGGGCCACGT13690.1103303543869009No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT13370.10775141257508146No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT13300.10718726905374594No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC13250.10678430939564916No Hit
AGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAA13110.10565602235297815No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC13080.10541424655812008No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC12960.1044471433786878No Hit
CGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGC12600.1015458338403909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)