FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192898
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC162320.7402077068792073No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA84530.3854716452840032No Hit
CCAGCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGG74990.3419675698550503No Hit
CTGCAAACCACATTGGCATCATTGGTGTCCCAGTAGTCATCACACACGGT74200.3383650311140783No Hit
AGCGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCAAACCGGGCA71620.3265997780106507No Hit
GTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCT68800.3137400827580672No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGA68380.31182480899704407No Hit
GTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCT68190.3109583756289622No Hit
GTCCTGAGCAGCGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCA67340.3070822263507012No Hit
GTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATG64160.2925808678743836No Hit
CCTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCC63550.2897991607452786No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG61310.2795843673531555No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTACTGTCGA57650.2628941245785258No Hit
CTGCTGTTGATGCACGAGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC56940.25965639988727246No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA54610.24903119068921584No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT54540.24871197839571196No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT54030.24638628882875535No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA53220.24269254657535372No Hit
GTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCAGC52850.2410052815954048No Hit
GGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCAGCTCCACAGATAG52150.23781315866036631No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA50710.23124650576543002No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT50540.23047127590977787No Hit
CTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCG49920.22764396702445808No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG49750.22686873716880585No Hit
CTCCACTCGGCCCTGACACCTGTCACCTCCATTCACCAGCCTCAGGGCCA49200.2243606405769899No Hit
CAGCGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCAAACCGGGC45920.20940326453852393No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG44230.20169656773821673No Hit
CCTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGG43570.19868685182803764No Hit
CCCTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCC41650.1899313146347892No Hit
CGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCCAGCTGCCTGCA41440.18897367775427767No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG40900.18651118291867658No Hit
GGCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGAT40430.1843679003765793No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG40090.1828174406652749No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT39990.18236142310312653No Hit
GCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCG39810.1815405914912595No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC38790.1768892123573463No Hit
CCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCAC38580.17593157547683477No Hit
CTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCCA38530.17570356669576057No Hit
CCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCC38530.17570356669576057No Hit
GGCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTC38420.1752019473773974No Hit
CCCAGGAGCCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCT36580.16681122423386768No Hit
AGCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGC34950.15937813797084954No Hit
GCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATG34780.15860290811519734No Hit
CCTGACACCTGTCACCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCA33600.15322190088184676No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC33380.1522186622451204No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG33290.15180824643918686No Hit
CCCTGACACCTGTCACCTCCATTCACCAGCCTCAGGGCCAAACTGGATTC33110.1509874148273198No Hit
GCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCAGCTCCACAGATAGG32910.15007537970302312No Hit
CGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCAAACCGGGCATT32710.1491633445787264No Hit
CAGCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGG32510.1482513094544297No Hit
GGCCCTGACACCTGTCACCTCCATTCACCAGCCTCAGGGCCAAACTGGAT32360.14756728311120718No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT31840.14519599178803574No Hit
CTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCACC31600.1441015496388797No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG31430.1433263197832275No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA31120.1419126653405676No Hit
GCCCTGACACCTGTCACCTCCATTCACCAGCCTCAGGGCCAAACTGGATT31080.14173025831570826No Hit
GTCCTGAACCCTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAG30920.14100063021627088No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC30260.13799091430609176No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC30090.13721568445043955No Hit
CTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATGGCCAC28750.1311050491176516No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT28650.13064903155550325No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT28510.1300106069684956No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT27560.1256784401280862No Hit
GGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCT27320.12458399797893016No Hit
GTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAG27310.12453839622271533No Hit
CACCAGCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGG27130.1237175646108483No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG26870.12253191894926257No Hit
GGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATGGCC26290.11988701708880213No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC26140.11920299074557959No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT25950.11833655737749772No Hit
GGACTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATGGCCACAGT25490.11623887659161529No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG25300.11537244322353342No Hit
CCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCA25170.11477962039274058No Hit
GGTCCTGCTGTTGATGCATGTGAGGTCGGCCAAGTATCTGGGCTGGGTGT25080.11436920458680704No Hit
GGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCA25050.11423239931816255No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA24980.11391318702465869No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC24920.11363957648736968No Hit
GTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACG24600.11218032028849495No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC24520.11181550623877627No Hit
GCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGCTG24370.11113147989555373No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC24200.11035625003990154No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC24180.11026504652747186No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC23670.10793935696051526No Hit
GGTTGGCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGC23660.10789375520430043No Hit
GCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTT23470.10702732183621856No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC23220.10588727793084766No Hit
GTTGGCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCA23080.10524885334383999No Hit
CTGGGGCTGACGTGGCCCAGCCACAGCCCAGCTGCCTGCAAACCACATTG22890.1043824199757581No Hit
GGTCTTACCAGTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGAC22580.10296876553309821No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG22580.10296876553309821No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA22320.10178311987151249No Hit
CCAGGAGCCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTC22230.10137270406557898No Hit
CTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGAT22070.1006430759661416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)