FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003490841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192898
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC119200.5435729340808373No Hit
CCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCC81850.3732503746184273No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT75640.3449316840090145No Hit
CAATGATGCCAATGTGGTTTGCAGGCAGCTGGGCTGTGGCTGGGCCACGT70790.32281483224481944No Hit
CCCAGCCCAGACACTTGGCCAACCTCACATGCATCAACAGCAGGATCTGA70360.3208539567275815No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC64940.29613780485914076No Hit
CATCTGCTCAGCTTCCCAGTCCCAGCCGACACCCAGCCCAGACACTTGGC63440.28929754142691544No Hit
CCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGC62270.2839621359497797No Hit
GTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAACTGTG60580.2762554391494725No Hit
CCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGCTCAGGACATG60500.2758906250997539No Hit
CATGAGTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAA58110.2649918053644082No Hit
CGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTC56650.25833394895704226No Hit
GGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAG54790.24985202230108286No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC47250.21546829811509702No Hit
GTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCC46830.21355302435407392No Hit
CCTGGGGCACCGTGTGTGATGACTACTGGGACACCAATGATGCCAATGTG44210.20160536422578704No Hit
AGTGGAGGTCCTATACCGAGGCTCCTGGGGCACCGTGTGTGATGACTACT43200.19699958684808871No Hit
GACCCATTGTCCTGGATGATGTGCGCTGCTCAGGACATGAGTCCTATCTG41610.18974890760992988No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA41240.18806164262998096No Hit
CAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAGGCTCCTGGGGCACCG41130.18756002331161778No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC40860.18632877589381722No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT40090.1828174406652749No Hit
CATGAAGACGCTGGTGTCATCTGCTCAGCTTCCCAGTCCCAGCCGACACC39890.1819054055409782No Hit
CAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGAT39320.1793061054367326No Hit
GGCTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGG38060.17356028415366334No Hit
GGCAGCTGGGCTGTGGCTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTT36690.1673128435522309No Hit
CCACAACTGTGGCCATCATGAAGACGCTGGTGTCATCTGCTCAGCTTCCC36480.16635520667171935No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC35630.16247905739345833No Hit
CTCTCCCACAACTGTGGCCATCATGAAGACGCTGGTGTCATCTGCTCAGC35580.16225104861238418No Hit
CCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGA35410.16147581875673195No Hit
CTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGAC35220.16060938538865008No Hit
CCCACAATGGCTGGCTCTCCCACAACTGTGGCCATCATGAAGACGCTGGT34720.1583292975779083No Hit
TCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCA34170.15582120098609237No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC34130.15563879396123304No Hit
GCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTG34050.15527397991151434No Hit
TGAAGACGCTGGTGTCATCTGCTCAGCTTCCCAGTCCCAGCCGACACCCA34030.1551827763990847No Hit
GGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGT33220.15148903414568302No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC33200.15139783063325335No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA32710.1491633445787264No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC32440.14793209716092587No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT32420.1478408936484962No Hit
CAGCAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGAC31880.14537839881289508No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT31430.1433263197832275No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC31420.14328071802701267No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30740.14017979860440385No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC30290.13812771957473624No Hit
CCCACAACTGTGGCCATCATGAAGACGCTGGTGTCATCTGCTCAGCTTCC30060.13707887918179507No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC29350.13384115449054174No Hit
GTAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAG28630.1305578280430736No Hit
CTGCTCAGCTTCCCAGTCCCAGCCGACACCCAGCCCAGACACTTGGCCAA28620.13051222628685874No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG27780.1266816787648126No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT27340.12467520149135984No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT27210.12408237866056698No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT26860.12248631719304773No Hit
CTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCG26490.12079905221309883No Hit
AGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAA26400.12038863640716532No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT25530.11642128361647464No Hit
CCTGGATCCTCACAGAGCTGACCACAACAGCCACTACGACTGAGTCCACT25460.11610207132297079No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG24910.11359397473115485No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT24470.11158749745770208No Hit
GGTGAATGGAGGTGACAGGTGTCGAGGCCGAGTGGAGGTCCTATACCAAG24460.11154189570148725No Hit
CACAACTGTGGCCATCATGAAGACGCTGGTGTCATCTGCTCAGCTTCCCA24370.11113147989555373No Hit
CCGTGTGTGATGACTACTGGGACACCAATGATGCCAATGTGGTTTGCAGG24170.11021944477125703No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC24110.10994583423396803No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA23950.10921620613453065No Hit
CCTGGATCCTCACAGAGCTGACCACAGCAGCCACTACAACTGCAGCCACT23650.10784815344808558No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT23550.10739213588593724No Hit
CCAATGATGCCAATGTGGTTTGCAGGCAGCTGGGCTGTGGCTGGGCCACG23310.10629769373678118No Hit
CAGGACATGAGTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCC23150.10556806563734383No Hit
GGCCGAGTGGAGGTCCTATACCGAGGCTCCTGGGGCACCGTGTGTGATGA23000.10488403929412128No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG22930.10456482700061745No Hit
GGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAGGCTCCTG22850.10420001295089877No Hit
CAGGACACGAGTCTTACCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCC22810.10401760592603942No Hit
CCACAATGGCTGGCTCTCCCACAACTGTGGCCATCATGAAGACGCTGGTG22790.10392640241360974No Hit
CGTGTGTGATGACTACTGGGACACCAATGATGCCAATGTGGTTTGCAGGC22730.10365279187632075No Hit
CTGGGACACCAATGATGCCAATGTGGTTTGCAGGCAGCTGGGCTGTGGCT22710.10356158836389107No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC22430.10228473918987568No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG22290.10164631460286798No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC22250.10146390757800866No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA22220.10132710230936415No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG22200.10123589879693447No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC21980.10023266016020808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)