FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320543
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA62670.4745775033452148No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT61180.46329426607085117No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG44190.33463507057324143No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG38960.29503015047597847No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC33840.25625822104997714No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG31440.23808387913153906No Hit
GGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGC30300.22945106672028098No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT29980.2270278211311559No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG28380.2149115931855305No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC28310.21438150821290938No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC26340.1994634025548581No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA26310.19923622328087762No Hit
CTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAG24880.18840734455447494No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA24000.18174341918438097No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG22680.17174753112924002No Hit
CCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTG22480.17023300263603686No Hit
CTCCATCCTTTTTTTCTCTGGCTATATTCAAAACAGAATCTTCCTCGTCA22010.1666738606770094No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT21680.16417488866322416No Hit
CCTAGATGTTTCTCCATCCTTTCTTTCTCTGGTTATATTTGAAAAAGAAT20450.15486053843002462No Hit
GGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAG19430.14713644311468843No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC18490.1400181591966335No Hit
CTCTGGTTATATTCGAAAAAGAATCTTTCTCATCACTTGTAGCCTTCAAG18450.13971525349799285No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG18430.13956380064867255No Hit
GTGGTTTCTGAGAAGACACTGTCCTAGATGTTTCTCCATCCTTTCTTTCT18110.13714055505954748No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT17850.13517166801838335No Hit
CTCTGGTTATATTTGAAAAAGAATCTTTCTCATCACTTGTAGCCTTCAAG17770.13456585662110207No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC17500.1325212431552778No Hit
GGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAA16950.12835628979896907No Hit
CTTTTTTTCTCTGGCTATATTCAAAACAGAATCTTCCTCGTCACTTGTAG16250.12305544007275794No Hit
CTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTG15970.1209351001822735No Hit
TGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAG15200.11510416548344128No Hit
GTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGC15120.11449835408616No Hit
TGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCG14960.11328673129159747No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT14960.11328673129159747No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT14950.11321100486693732No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC14860.11252946704499588No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA14780.1119236556477146No Hit
CTGGTGGTTGCTCAGAAGACACTGTCCTAGATTTTTCTCCATCCTTTTCT14740.11162074994907398No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA14630.11078775927781223No Hit
CTAGTTTTTTCTCCATGCTTTTTTCCTCTGGCTATATTCAAAACAGAATC14600.11056058000383176No Hit
GCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGT14450.10942468363392938No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA14390.10897032508596842No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT14350.10866741938732778No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC14280.10813733441470669No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA14150.10715289089412461No Hit
AGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCA14070.10654707949684335No Hit
AGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGAT14020.10616844737354254No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG13890.10518400385296049No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAG13840.10480537172965969No Hit
GGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGG13670.103518022510437No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC13660.10344229608577682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)