FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320543
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68890.5216793394838336No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA52680.3989268051097162No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC43480.32925849442237015No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT42390.3210043141344129No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC41500.3142646623396588No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT38890.29450006550335733No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT38580.29215254633889237No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT35570.2693588925161846No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG33800.25595531535133653No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC32280.24444489880299242No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG31960.24202165321386732No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT31490.23846251125483986No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT27120.2053700636783505No Hit
GGAAGATTCTGTTTTGAATATAGCCAGAGAAAAAAAGGATGGAGAAAAAT24820.18795298600651397No Hit
GAAAGATTCTTTTTCAAATATAACCAGAGAAAGAAAGGATGGAGAAACAT24280.1838637590748654No Hit
GAAACATCTAGGACAGTGTCTTCTCAGAAACCACCAGCCTTGAAGGCTAC20940.15857113323837238No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC20320.15387609490944257No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC20300.15372464206012224No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20040.1517557550189581No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC19920.1508470379230362No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC19570.14819661305993065No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC19560.1481208866352705No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC19060.14433456540226255No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC18460.13979097992265305No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT18280.13842790427877016No Hit
CCTGGATCCTCACAGAGCTGACCACAACAGCCACTACGACTGAGTCCACT18270.13835217785411No Hit
TTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAG17260.13070380896343398No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT16690.12638740275780494No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA16560.1254029592372229No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT16000.12116227945625398No Hit
CAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTT15790.11957202453839065No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT15780.11949629811373048No Hit
ACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACT15220.1152556183327616No Hit
CAGCTGGAAGATGGTCGTACCCTGTCTGACTACAACATCCAGAAAGAGTC14960.11328673129159747No Hit
CTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGC14830.11230228777101542No Hit
CAACAACATTCTATAACTGATAACTCCCTGAGCCTCAAGACACCTCCCGA14310.10836451368868714No Hit
AGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCC14150.10715289089412461No Hit
CACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCA13990.10594126809956209No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC13780.10435101318169873No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA13690.10366947535975732No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC13690.10366947535975732No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG13650.10336656966111668No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC13470.10200349401723381No Hit
CCACCAGCCACCTCCTCCAACTCAACAACATTCTATAACTGATAACTCCC13390.10139768261995255No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC13300.10071614479801112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)