FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216724
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA113370.3524393140350245No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG80190.24929089346801278No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT73780.22936378750554914No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG64480.20045238571913537No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA62180.1933022540945384No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT58970.18332315734890528No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG56910.17691912641557062No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG53640.16675350449712192No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51590.16038056109258986No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC49730.15459828073530713No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT48160.14971753871329962No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC46300.14393525835601687No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT46070.14322024519355717No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC43840.1362877262705784No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.12627754199614266No Hit
GCTGGTTGTAAGGGGAGAGATTGTGCTGGTTGTAGAAGCAGAGGTTGTGC40400.12559361636248556No Hit
GGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGTGGGAA39550.12295117641426495No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT38660.12018438635083395No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA38650.12015329882203137No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA38240.11887871014112494No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG37350.11611192007769396No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC36680.11402905564792006No Hit
GCTGGTTGTAGGGGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGC35460.11023637713400343No Hit
GTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTG32840.10209144458772342No Hit
GGCAGAGTTTGTGCTGGTTGTAGGTGCAGAGGTTATGCTGGTGGTGGGAA32570.10125208131005334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)