FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991440
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA165250.5524095418928676No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA150860.504305618698687No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT149540.499893028106865No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA125220.41859438932420506No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG124150.4150175166475009No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT117260.39198513090685416No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT116470.389344262295082No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG113060.37794506993287513No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT111400.37239590297649294No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT101530.3394017596876421No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA101050.3377971812906159No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG101040.3377637525740112No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT95450.3190770999919771No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG94060.3144305083839221No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG86730.28992725911266815No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC79200.2647554355093199No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT65450.21879095017784078No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC63810.21330864065466798No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC63590.21257320888936432No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG62640.20939748081191667No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA62440.2087289064798224No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA61690.20622175273446902No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC60230.20134116011018105No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC57390.1918474045944428No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG56470.1887719626668093No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG52370.17506618885887731No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA52160.17436418581017837No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC50880.17008531008477523No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC50190.1677787286390501No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC49430.16523814617709198No Hit
CTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAAT48880.1633995667638328No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG47380.158385259273126No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC45480.1520338031182307No Hit
CGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTT45110.15079694060385632No Hit
GTCCTTTTTAGTTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT44550.14892493247399247No Hit
CTGAAGGAGTCGAATAACATCTATCCAGTGAGTCCTGCAAGACTTCAGGC44290.14805578584226994No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT42670.1426403337523066No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG42500.14207204557002648No Hit
GTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAA41610.13909688979220708No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG40770.1362888775974113No Hit
CACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGA40340.13485144278340866No Hit
CAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGC39870.13328029310298717No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT39700.1327120049207071No Hit
CTTGAGATTATCATCGGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG39290.13134142753991387No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA39130.1308065680742385No Hit
TTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAA39120.1307731393576338No Hit
CTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTG38800.129703420426283No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT38460.12856684406172278No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA38290.12799855587944267No Hit
CAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGC38100.12736341026395315No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT38030.12712940924772015No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT37900.1266948359318589No Hit
CTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTA37850.12652769234883535No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG37180.12428796833631962No Hit
GTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTGCTGAGCC36920.12341882170459713No Hit
AGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGAT36610.12238253148985105No Hit
CTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCA36300.12134624127510497No Hit
CTCCAATGAGTAAACAGCACTGCTGTAGGGCTGGCCTAAGTCAGGCAGTT36190.12097852539245313No Hit
GTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCC36160.120878239242639No Hit
CTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAGGAGT35900.12000909261091648No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC35880.11994223517770705No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC35650.11917337469579867No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG35090.1173013665659348No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA34990.1169670793998877No Hit
TGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAG34710.11603107533495573No Hit
CTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGT34440.11512849998662851No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC34130.11409220977188243No Hit
CTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG33390.11161848474313374No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA33170.11088305297783008No Hit
ATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAGG32570.10887732998154735No Hit
GTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGT32300.10797475463322014No Hit
CTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATC32000.10697189313507877No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC31770.10620303265317038No Hit
CCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTG31390.10493274142219132No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT31330.10473216912256304No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC31070.10386302249084053No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG30920.10336159174176986No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG30820.10302730457572273No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG30250.10112186772925415No Hit
GTCTTCTTCCACTTCTTGGTACTTTTCAATTTCATCCATGTCAACAGCCA30230.1010550102960447No Hit
CGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTG30130.1007207231299976No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG29980.10021929238092692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)