FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003490863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481974
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT99830.40222016830152124No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC92780.37381535825919204No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC87600.3529448737174523No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT78050.3144674360005383No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC67040.2701075837216667No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC56410.227278770849332No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC53130.21406348334027672No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC52480.2114446001448847No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA48020.19347503237342534No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG45330.18263688499557207No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG44530.17941364413970493No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA44020.17735882809408962No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT42180.16994537412559518No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT40590.16353918292455924No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT39750.16015478002589872No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC39460.15898635521564689No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT37830.1524190019718176No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC35090.1413794020404726No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC34710.13984836263393574No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT33830.13630279769248188No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT33380.1344897247110566No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG32950.13275723275102802No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT32520.13102474079099943No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC32410.1305815451733177No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC32010.1289699247453841No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG29320.11813177736753083No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT29120.11732596715356405No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA29000.11684248102518399No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC28690.11559347519353547No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG28140.1133774971051268No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA26820.10805914969294601No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT25750.10374806504822372No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC25450.10253934972727352No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA25340.1020961541095918No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC25050.10092772929933995No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT24940.10048453368165823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)