FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003490867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415068
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT46630.3295248002216148No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT46500.32860611645518095No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC46280.32705142085044675No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC44980.3178645831861084No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT38120.269386347511215No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT35150.24839795684730345No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA33770.23864577532669806No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC29790.21051991847741594No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG28080.19843569354970925No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC26650.18833017211893704No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC26400.18656347256810274No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA22600.1597096393954213No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC21200.14981612191074917No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT20610.1456467109707802No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC20560.14529337106061335No Hit
CCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGT20550.14522270307857996No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT20290.14338533554571228No Hit
TATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGG20070.1418306399409781No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA19910.14069995222844414No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC19870.14041728030031064No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC19810.13999327240811044No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG19590.13843857680337623No Hit
CCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGAT19110.13504651366577436No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC17990.12713169967803667No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT17990.12713169967803667No Hit
AGACAGTGGACTGTTATGGGTGTAGGTGAATTGGCTTATTTTGTCTGTCC17590.12430498039670179No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA17250.12190226900756712No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAC17130.12105425322316665No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT17040.1204182413848663No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA16960.11985289752859934No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA16810.11879287779809876No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG16520.11674350631913094No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG15960.11278609932526211No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT15370.10861668838529316No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC14960.10571930112192487No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT14890.10522462524769129No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA14880.1051539572656579No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT14760.10430594148125744No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT14720.10402326955312395No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC14480.10232723798432303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)