FastQCFastQC Report
Fri 31 May 2024
EGAF00003490876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003490876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23738485
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length101-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC427780.18020526583731017No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG423290.17831382247013658No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA294630.12411491297780798No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG292700.12330188720973559No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT264030.11122445261355138No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG259950.10950572456498381No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG240630.1013670417467669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)