FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003491272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645275
Sequences flagged as poor quality0
Sequence length126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGT43400.2637856893224537No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA42340.25734299737126015No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA38140.23181535001747428No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA32500.19753536642810474No Hit
CTCCATCCTTTTTTTCTCTGGCTATATTCAAAACAGAATCTTCCTCGTCA32110.19516494203096746No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGT30930.18799288872680858No Hit
GGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAG30720.1867165063591193No Hit
CTCCATCCTTTCTTTCTCTGGTTATATTTGAAAAAGAATCTTTCTCATCA29830.1813070763246266No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT28330.17219005941256021No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC27190.16526112655938976No Hit
GAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGT24440.14854659555393476No Hit
AGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTG23760.14441354788713134No Hit
GGGTTGTCTTGGATACAGAAAGATGGCTTGTGCTGGTCTTTGAAACAGGG23060.1401589399948337No Hit
GTGCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGT22820.13870021728890308No Hit
AGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAG22600.13736305480846667No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG22490.13669447356824846No Hit
GGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGG22260.13529653097506497No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG21830.13268298612693927No Hit
GGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCT21740.1321359651122153No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA21740.1321359651122153No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA21370.12988710094057224No Hit
CTCTGGTTATATTTGAAAAAGAATCTTTCTCATCACTTGTAGCCTTCAAG21100.1282460378964003No Hit
CTCGTCAGTTGTAGCCTTCAAGGCTGGTTGTTTCTGAGAAGACACTGTCT21070.12806369755815897No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG20750.12611873395025147No Hit
GTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCC20660.1255717129355275No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT20570.1250246919208035No Hit
GCCTTCCTTGTCTTGGATCTTTGCCTTGACATTCTCAATGGTGTCACTCG20380.12386986977860845No Hit
GGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGG20320.1235051891021258No Hit
CTCCATCCTTTTTTTCTCTGGTTATATTCGAAAAAGAATCTTTCTCATCA20250.12307972831289603No Hit
GGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCAT20090.12210724650894228No Hit
CTCCATGCTTTTTTCCTCTGGCTATATTCAAAACAGAATCTTTCTCGTCA19610.11918980109708102No Hit
GCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACC19390.11785263861664463No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG19370.1177310783911504No Hit
GTCCATCTTCCAGCTGTTTCCCAGCAAAGATCAACCTCTGCTGATCAGGA19060.11584689489599004No Hit
CTGGTGGTTGCTCAGAAGACACTGTCCTAGATTTTTCTCCATCCTTTTCT18140.11025512452325599No Hit
CCGTCCTTTATTTCTGTGGCTATAATCGAAACAGAATCTTCCTCGTCAGT18090.10995122395952045No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC17590.10691221832216499No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTAAGACGGAGCACCAGGTGCAG17360.10551427572898149No Hit
CCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC17270.1049672547142575No Hit
GCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGC17000.10332619167008555No Hit
CTCGTCACTTGTAGCCTTCAAGGCTGGTGGTTTCTGAGAAGACACTGTCC16680.10138122806217806No Hit
CTTTTTTTCTCTGGCTATATTCAAAACAGAATCTTCCTCGTCACTTGTAG16470.10010484569448876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)