FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003491273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645275
Sequences flagged as poor quality0
Sequence length126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT41100.2498062633906186No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA31150.189330051207245No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT29430.1788758718147422No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA28590.17377034234398503No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC27490.16708452994180303No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27390.16647672881433195No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC27360.16629438847609063No Hit
CCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26220.15936545562292018No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC26070.15845375393171354No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA25870.15723815167677135No Hit
GGAAGATTCTGTTTTGAATATAGCCAGAGAAAAAAAGGATGGAGAAAAAT25330.15395602558842747No Hit
CANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25250.1534697846864506No Hit
CTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC24140.1467231921715215No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC21820.13262220601419217No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG21760.13225752533770951No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC21540.1309203628572731No Hit
CTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT21080.12812447767090607No Hit
CTCTCGAGGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATC20710.12587561349926304No Hit
CCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCC20600.12520703225904484No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC20600.12520703225904484No Hit
ACCAGCCTTGAAGGCTACAAGTGACGAGGAAGATTCTGTTTTGAATATAG20020.12168178571971251No Hit
AGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19960.12131710504322986No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG19450.11821731929312729No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC19130.11627235568521979No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT19010.11554299433225448No Hit
CCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTC18860.11463129264104785No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA18820.11438817219005942No Hit
GAAAGATTCTTTTTCAAATATAACCAGAGAAAGAAAGGATGGAGAAACAT18260.11098448587622131No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC17890.10873562170457826No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC17880.10867484159183115No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA17870.10861406147908403No Hit
TGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTA17700.10758079956238319No Hit
GCTACAAGTGATGAGAAAGATTCTTTTTCAAATATAACCAGAGAAAGAAA16970.10314385133184421No Hit
GGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16810.10217136952789048No Hit
GANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16800.10211058941514337No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC16670.10132044794943094No Hit
CCGTCTCAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGA16640.1011381076111896No Hit
CTTTGCCGGAAAACAGCTGGAAGATGGTCGTACCCTGTCTGACTACAACA16530.10046952637097141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)