FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003491286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594219
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT94500.26292220924768356No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG83100.23120460940193127No Hit
GGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGCTAGTGACAGGAAGAGG75520.21011518775010649No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG75020.20872406494985418No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG71970.20023821586831522No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA61810.17197060056718858No Hit
GTGTGACCTGAGGATGCTGAGGAAGGGCTGGTGACAGGAAGAGGGGTGGT58860.16376297604570006No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA54180.1507420666353386No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT52230.14531668771435463No Hit
GGCCTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG51620.14361951789804683No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG49810.13858365336113354No Hit
GGTGGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGG49780.1385001859931184No Hit
GTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGT48900.13605180986467436No Hit
GGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGG48540.13505020144849272No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA48390.13463286460841703No Hit
GGGCTAGTGACAGGAAGAGGCGTGGTGTCACCTGTGGATACTGAGGAAAG47500.13215666602396792No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGAGGTGG47060.1309324779597459No Hit
GGTGGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGG46790.13018127164760968No Hit
GGCGTGGTGTCACCTGTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGG46260.12870668147934225No Hit
CTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCT46000.12798329762321103No Hit
CTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCT44470.12372646185443903No Hit
GGGGTGGTGTCACCTGTGGATGCTGAGGAAAGGCTGGTGACAGGAAGAGG43430.12083292642991426No Hit
GGCGTGACCTGTGGATGCTGAGGAAGGGCTAGTGACAGGAAGAGGCGTGG42470.11816197065342986No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGTAGATGCTGAGGAAGGGC42160.11729947451727343No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGAGGATGCTGAGGAAGGGC41770.11621439873307664No Hit
GGATGCTGAGGAAGGGCTAGTGACAGGAAGAGGCGTGGTGTCACCTGTGG41580.11568577206898079No Hit
GCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTGT39720.11051079525204224No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC38570.10731121281146196No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA38450.1069773433394014No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC37960.10561404299515417No Hit
GGATGCTGAGGAAGGGCTAGTGACAGGAAGAGGCATGGTGTCACCTGTGG37260.10366647107480095No Hit
GGGTGGTGTCACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGG37070.10313784441070507No Hit
GGATACTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGG36930.10274833002663444No Hit
GGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGCGTGACCTGTGG36870.10258139529060416No Hit
GGGGTGGTGTCACCTGTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGG36520.10160760933042756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)