FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003491291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055895
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN367001.7851106209217884No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA73040.3552710619948976No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT60970.29656183803161157No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT42670.20754951006739158No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG37930.18449385790616737No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC37630.18303463941495068No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC37220.18104037414362115No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC36540.17773281223019657No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC36330.17671135928634488No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC34660.16858837635190513No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC34420.16742100155893175No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT33880.16479440827474165No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC33140.16119500266307377No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT31890.1551149256163374No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT30030.14606777097079374No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC28870.14042545947142243No Hit
GCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCG28840.14027953762230075No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT28310.13770158495448453No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT26600.12938403955454922No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT26150.12719521181772414No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT26020.12656288380486358No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT25650.12476318099902961No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA25500.12403357175342125No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA25480.12393629052067348No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA24710.12019096305988389No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG24260.11800213532305881No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC24100.11722388546107655No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC24100.11722388546107655No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT24040.11693204176283323No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA23850.1160078700517293No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC23450.11406224539677366No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT23180.11274894875467861No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC22910.11143565211258358No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC22670.1102682773196102No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC22620.11002507423774074No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG22290.10841993389740234No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT21510.10462596582023888No Hit
CCACCACCGAGACCACATCCCACAGTACTCCCAGCTTCACTTCTTTGATC21430.10423684088924776No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA21220.10321538794539604No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT21060.10243713808341379No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG20930.10180481007055321No Hit
GCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT20820.1012697632904404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)