FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003491590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6390178
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA157112124.586498216481605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA99325215.543416787450992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC77470712.123402509288473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA1197821.8744704764092643Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA885071.3850474900699166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGTCCTGACATAAGATCGGAAGAGCACACGTCTGA859851.3455806708357732Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA429690.6724225835336668Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA395200.6184491261432781Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA391560.6127528841919584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA345520.5407048129175744Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA318000.4976387199229818Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA313150.49004894699333884Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACCAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC310740.486277534053042Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA285270.44641948941015414Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA277570.4343697468208241Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA272330.4261696622535397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC271980.4256219466812975Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACTTTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA258930.40519998034483545Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACTGACATAAGATCGGAAGAGCACACGTCTGAACT250280.3916635812022764Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA232070.36316672242932824Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTCTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA219680.34377759117195167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC219210.34304208740351205Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA199770.3126203996195411Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA192690.3015408960438974Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTATATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA187290.29309042721501655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTTTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA179000.2801173926610495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACTGACATAAGATCGGAAGAGCACACGTCTGAA178760.2797418162686548Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT175920.27529749562531747Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT173640.27172951989756783Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGATCTGACATAAGATCGGAAGAGCACACGTCTGAA171900.2690065910527062Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA167980.2628721766435927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT155420.2432170121082699Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA152120.23805283671284272Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA149540.23401539049459968Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA147940.23151154787863498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT146130.22867907591932493Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTCTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA120360.18835156078594367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA120120.18797598439354898Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA118910.18608245341522567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA118490.18542519472853494Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCCCTTATTTATTTATTTTATTTTATTTTAGA117150.18332822653766453No Hit
AGAGGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA116650.18254577572017558Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTATATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA105710.16542575183351702Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTACATAAGATCGGAAGAGCACACGTCTGAA103370.16176388200766864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGACT103090.16132570954987482Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAGAGGTACATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA102560.16049631168333653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCGACATAAGATCGGAAGAGCACACGTCTGAACT99340.15545732841870757Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGATCTGACATAGATCGGAAGAGCACACGTCTGAA98020.15339165826053672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT96240.150606133350276Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTAAGGACCTACATAAGATCGGAAGAGCACACGTCTGAACT95150.14890039056815005Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGATCTGACAAAGATCGGAAGAGCACACGTCTGAA94480.14785190647271484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACAAAGATCGGAAGAGCACACGTCTGAA92970.14548890500389816Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCGACATAAGATCGGAAGAGCACACGTCTGAA87710.13725752240391426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCGACATAAGATCGGAAGAGCACACGTCTGAA85670.13406512306855928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGAAGAGCACACGTCTGAACT85670.13406512306855928Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGAGAGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT83070.12999637881761666Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGACCTGACATAGATCGGAAGAGCACACGTCTGAA82530.12915133193472858Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA81320.1272578009564053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA77900.12190583736478076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACTAAGATCGGAAGAGCACACGTCTGAA76900.12034093572980283Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGAATAAGATCGGAAGAGCACACGTCTGAA74030.11584966803741617Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGAAGAGCACACGTCTGAA72650.11369010378114662No Hit
AGAGAGGTACTATTAGGATCCCTACACGACGCTCTTCCGATCTAGAGAGG70980.11107671805073349Illumina Single End PCR Primer 1 (96% over 26bp)
GGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA70970.11106106903438372Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGATCCTGACATAAGATCGGAAGAGCACACGTCTGA70110.10971525362830267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGATCTGACTAAGATCGGAAGAGCACACGTCTGAA68150.10664804642374595Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA67990.10639766216214948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA67320.10534917806671426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTACATAAGATCGGAAGAGCACACGTCTGAA66420.10394076659523413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)