FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003491591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7242904
Sequences flagged as poor quality0
Sequence length126
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA152401821.04153251237349Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA118090116.30424757804328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC104045314.365135862631895Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA1269201.7523358034291219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA963441.3301846883515231Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGTCCTGACATAAGATCGGAAGAGCACACGTCTGA811851.1208901843790833Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGTGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA526460.7268631477098136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA418780.5781934980775667Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC418040.5771718084348487Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACCAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC398410.5500694196692376Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA395420.5459412412479856Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA391070.5399353629428196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA366210.5056121135942158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC344860.4761349867401252Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACTAAGGACTGACATAAGATCGGAAGAGCACACGTCTGAACT333570.4605473163802806Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA331160.45721992173305076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA330610.45646055780940903Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA324520.44805232818217666Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA313190.4324094313551581Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA271620.37501532534464077Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTTTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA251640.34742970499125764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA239140.33017143399940135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA230420.31813206415548234Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT219950.30367653637270353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTCTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA208120.2873433087060107Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT206870.2856174816068251Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT203020.28030193414133336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTTTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA197270.27236312948507946Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACTGACATAAGATCGGAAGAGCACACGTCTGAA194660.2687596025019799Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT189490.2616215816197481Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA188780.2606413118274107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGACT183230.2529786395070265Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAGAGGTACTATTAGATCTGACATAAGATCGGAAGAGCACACGTCTGAA176690.24394911212408726Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTATATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA176170.24323116805082604Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA174630.24110494906462934Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGAAGAGCACACGTCTGAACT168260.23231013416717936Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGAGAGGTACTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA156250.2157283873982038Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA143670.19835966347199965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGAAGAGCACACGTCTGAA142100.19619202463542246No Hit
AGAGAGGTCTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA141730.19568117981406352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCGACATAAGATCGGAAGAGCACACGTCTGAACT133370.18413884817471005Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTAAGGACCTACATAAGATCGGAAGAGCACACGTCTGAACT123550.1705807504835077Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTATATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA123370.17033223138122497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCCCTTATTTATTTATTTTATTTTATTTTAGA117530.16226916717382972No Hit
AGAGAGGTACTATAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA116960.16148219001660108Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA116410.1607228260929594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTACATAAGATCGGAAGAGCACACGTCTGAA116100.16029482097236136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT114780.15847234755562134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA113290.15641516165339206Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA112160.15485501395572826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACAAAGATCGGAAGAGCACACGTCTGAA109180.1507406421512697Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAT105400.14552174100333237Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGAGAGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT102750.14186298755305884Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA102230.14114504347979762Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA99660.13759674296387195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCGAACT98720.13629892098528437No Hit
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGACACACGTCTGAACT98320.13574665631354496No Hit
AGAGAGGTACTATTAGGACCGACATAAGATCGGAAGAGCACACGTCTGAA98040.13536007104332737Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACATAGATCGGAAGAGCACACGTCTGAA97020.1339517961303919Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA96050.13261255430142385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC91840.12679996863136664Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA91780.12671712893060572Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATGGAAGAGCACACGTCTGAACT91650.12653764291229042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGGTACTATTAGGACCTGACTAAGATCGGAAGAGCACACGTCTGAA90640.12514317461614846Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTGACATAGATCGGAAGAGCACACGTCTGAA90000.1242595511413654Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCCCTACACGACGCTCTTCCGATCTAGAGAGG89670.1238039327871804Illumina Single End PCR Primer 1 (96% over 26bp)
AGAGAGGTACTATTAGGACCTGAATAAGATCGGAAGAGCACACGTCTGAA85970.11869548457359093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA84270.11634835971869846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCGAA83830.11574086857978512No Hit
AGAGAGGTACTATTAGGATCGACATAAGATCGGAAGAGCACACGTCTGAA83600.11542331639353497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGCTGAACT83040.1146501458530998No Hit
AGAGAGGTACTATTAGGATCTGACAAAGATCGGAAGAGCACACGTCTGAA82990.11458111276913237Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGACACACGTCTGAA81300.11224779453103341No Hit
AGAGAGGTACTAAGGACCTGACATAAGACGGAAGAGCACACGTCTGAACT80120.11061861374940217Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAGAGGTACTATTAGGACCTGACATAAGATGGAAGAGCACACGTCTGAA80110.11060480713260869No Hit
AGAGAGGACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA78880.10890659326701002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAATCGGAAGAGCACACGTCTGAACT77550.10707031323347652Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAGAGGTACTAAGGACCTGACAAAGATCGGAAGAGCACACGTCTGAACT77440.10691844044874818Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACCTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA77390.10684940736478075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACATAAATCGGAAGAGCACACGTCTGAA75230.10386717813738798Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGAGAGGTACATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA74130.10234845029010464Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGACATAAGACGGAAGAGCACACGTCTGAA73850.10196186501988706Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)