FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003491593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6902670
Sequences flagged as poor quality0
Sequence length126
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA179682726.03089818867192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA96658414.003045198452194Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC6462029.361623835414413Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACTATTAGGTCCTGACATAAGATCGGAAGAGCACACGTCTGA921711.3352948931355548Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA625820.9066346790444857Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA494810.7168385566744462Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA480860.696628985595429Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCCCTTATTTATTTATTTTATTTTATTTTAGA460590.6672635371530147No Hit
AGTGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA408530.5918434460868041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA393080.5694608028487527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA356000.5157424590774294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA338990.4910998207939827Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA333990.48385624693053564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTTTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA332690.4819729177260393Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGATCTGACATAAGATCGGAAGAGCACACGTCTGAA313820.45463566996539023Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACCAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC296280.429225212852418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTCTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA278420.40335116701218515Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA256490.3715808520471064Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTATATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA252040.36513407130863856Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACTGACATAAGATCGGAAGAGCACACGTCTGAA250290.3625988204564321Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA246370.3569198585474896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACTGACATAAGATCGGAAGAGCACACGTCTGAACT233860.33879643674114507Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA212310.3075766333896883Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTTTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA196650.2848897600493722Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA195020.28252835496988843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGATCGACATAAGATCGGAAGAGCACACGTCTGAA167610.24281908305047178Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT155720.2255938644031947Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA155680.22553591581228713Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA155020.22457976406231211Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA153470.22233425616464353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA153320.22211694894874015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA147010.21297555873306995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA146250.21187453550582602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT142640.20664467517641727Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACCTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA137250.19883610255162135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTCTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA133170.19292534627904853Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTATATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA131680.1907667612677413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT130750.18941945652914016Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA130040.1883908690405307Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGATCTGACAAAGATCGGAAGAGCACACGTCTGAA129730.187941767460997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA128460.1861018996996814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTACATAAGATCGGAAGAGCACACGTCTGAA127560.18479805640426097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTGACATAGATCGGAAGAGCACACGTCTGAA126560.18334934163157154Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA117740.17057167733645098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT116340.16854347665468578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGACCGACATAAGATCGGAAGAGCACACGTCTGAA114170.16539976559794978Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTACATAAGATCGGAAGAGCACACGTCTGAA111610.1616910557798649Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA106640.15449094335959854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGACCTGACAAAGATCGGAAGAGCACACGTCTGAA100580.14571173183710073Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTGACTAAGATCGGAAGAGCACACGTCTGAA97280.14093097308722566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGACT93930.13607777859871614Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAGGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA90100.1305292010193157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT87440.12667561972396188Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGAAGAGCACACGTCTGAA87070.1261395952580668No Hit
AGAGAGGTACTAAGGACCGACATAAGATCGGAAGAGCACACGTCTGAACT86560.1254007507239952Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGACCTGACATAGATCGGAAGAGCACACGTCTGAA86090.12471985478083118Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTGAATAAGATCGGAAGAGCACACGTCTGAA85960.12453152186038156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA85610.12402447168994027Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACCTGAATAAGATCGGAAGAGCACACGTCTGAA85500.12386511306494442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTACATAAGATCGGAAGAGCACACGTCTGAACT83210.12054755623548569Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGATCCTGACATAAGATCGGAAGAGCACACGTCTGA82510.11953345589460311Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT81550.11814268971282126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGACCTGACTAAGATCGGAAGAGCACACGTCTGAA79390.11501346580381215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA78350.11350680244021516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC77000.11155103749708446Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGAAGAGCACACGTCTGAACT75770.10976911832667649Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGAGAGGTACATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA75560.10946488822441171Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA75440.10929104245168898Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAAGACCTGACATAAGATCGGAAGAGCACACGTCTGA72380.1048579752472594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGATCATGTCAGCATTTAGTGATGTGGGACTCAGA71340.10335131188366241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)