FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003491594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6362693
Sequences flagged as poor quality0
Sequence length126
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA124237119.525867427518506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC82255612.927796453482825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA4866557.648569560090358Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTATTAGGATCCCTTATTTATTTATTTTATTTTATTTTAGA1457422.2905709893593795No Hit
AGAGAGGTACTATTAGGTCCTGACATAAGATCGGAAGAGCACACGTCTGA603430.9483877345645939Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACCAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC493380.7754263799935028Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACTAAGGACTGACATAAGATCGGAAGAGCACACGTCTGAACT326850.5136975805684795Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA318700.5008885388623967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCATGTCAGCATTTAGTGATGTGGGACTCAGA299620.4709012363161322No Hit
GAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA271540.42676897973861067Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA268560.4220854282927056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTACTAAGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT264200.4152329838953412Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA264000.41491865158353547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT229710.3610263767244467Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTAGGATCCTCCCACCCCAGCCTCCTGAGTAGCTAGGA220640.3467714063840578No Hit
AGAGAGGTACTTTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA214810.33760861949492144Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA208610.32786431782894443Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGATCTGACATAAGATCGGAAGAGCACACGTCTGAA203920.32049322511710054Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA179240.2817046178402761Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT172630.2713159349350974Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTCTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA166240.26127301757290505Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCAAAATGAGACTATTTCTATCACACTCCAGA157880.24813392693942643No Hit
AGAGAGGTATATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA149270.2346019209161907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT142350.22372602292771315Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATTGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA139560.2193410871780235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT139530.2192939373312527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA130770.20552618207416262Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA130050.20439458575166208Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGACTGACATAAGATCGGAAGAGCACACGTCTGAA129150.20298009034853637Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTTTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA128090.20131412909596613Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCCACCCACCTCAGCCTCCCAAAGTGTTGGGA126070.1981393727467285No Hit
AGAGAGGTACTATAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGA122780.19296860621752454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAACT121350.19072113018811376Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTAAGGACCGACATAAGATCGGAAGAGCACACGTCTGAACT113660.17863505279918424Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA113650.17861933618359396Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTACATAAGATCGGAAGAGCACACGTCTGAACT103750.16305988674921137Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGGTACTATAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA102890.16170825780844683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGAGGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA102330.16082812733539087Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGTACTATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA98680.15509156264493668Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACCTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA98490.15479294694872123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA97000.1524511712257687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAAC96460.1516024739838933Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGGTACTATTAGGATCGACATAAGATCGGAAGAGCACACGTCTGAA94560.14861631702173908Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTGACAAAGATCGGAAGAGCACACGTCTGAA93240.14654172376382138Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCACCTGAGGTCAGGAGTGACCAGCCCGGCCA91250.14341411726135458No Hit
AGAGAGGTACTATTAGGATCTGACATAGATCGGAAGAGCACACGTCTGAA90810.14272258617538203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA89910.14130809077225634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA88970.1398307289067695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA87340.13726892056555298Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTAAGGACCTGACATAAGATCGGAAGAGCACACGTCTGACT86040.13522576053881588Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAGAGGTACTATTAGGATCGGAAGAGCACACGTCTGAACTCCAGTCACA85020.13362266574860676Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGAGAGGTACATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA83000.1304479093993691Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGAA81220.1276503518242983Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTACATAAGATCGGAAGAGCACACGTCTGAA73100.11488845996498653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGAGGTACTATTAGGATCTGACATAAGATCGGAAGAGCACACGTCTGA72850.11449554457522938Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGGTATATTAGGACCTGACATAAGATCGGAAGAGCACACGTCTGAA68830.10817746510793465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTGACTAAGATCGGAAGAGCACACGTCTGAA68110.1070458687854341Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGGTACTATTAGGATCTTGAGGACAGGGCCTGGCCCAGCACTGTGG68020.10690441924512152No Hit
AGAGAGGTACTATTAGATCCCTTATTTATTTATTTTATTTTATTTTAGAG67380.10589855584734326No Hit
AGAGAGGTACTATTAGGATCTAAGGACCTGACATAAGATCGGAAGAGCAC67170.10556850691994725No Hit
AGAGAGGTACTAAGGACCTGACAAAGATCGGAAGAGCACACGTCTGAACT66670.10478267614043299Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)