FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003491595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5998619
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA82810013.804844081612785No Hit
CCTCTCCCCTGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA451060.7519397381297261No Hit
CCGCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA401500.6693207219861772No Hit
CCTCTCCCCTGGTGCTGATCGTGACTGGGTTTCCAGCCAGGAGAAAGATC399420.6658532572247046No Hit
CCTCGCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA357050.5952203332133613No Hit
CTCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA323600.539457498467564No Hit
CCTCTCCCCTGGTGCTGATCATGGGTTTCCAGCCAGGAGAAAGATCGGAA259110.4319494203582524No Hit
CCTCTCCCCTGGTGCTGATCGGGTTTCCAGCCAGGAGAAAGATCGGAAGA254990.425081172849951No Hit
CCTCTCCCCTGGTGCTGATCAGGGTTTCCAGCCAGGAGAAAGATCGGAAG236350.39400735402598497No Hit
CCTCTCCCCTGGTGCTGACCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA235030.39180684754274275No Hit
CCTCTCCCCTGGTGCTGATCCCCATACCTATTTCACAGTGCTGCCCTCCG202270.3371942775495493No Hit
CCTCTCCCCTGGTGCTGATCGGAGGTCCTTGGGCAGCATCAGTCAAAGCA200550.33432695091987No Hit
CCTCTCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA194520.3242746372123317No Hit
GCTCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA162830.27144581111085736No Hit
CCTCTCCCCTGGTGCTGATCCAGGGTTTCCAGCCAGGAGAAAGATCGGAA141770.23633773040094727No Hit
CCTCTCCCCTGGTGCTGATCATTCAGGGTTTCCAGCCAGGAGAAAGATCG119790.199695963354232No Hit
CCTCCCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA114500.1908772669176022No Hit
CCGCGCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA107350.17895785680004014No Hit
CCTCTCCCCTGGTGCTGATCCAGTCACGGGTTTCCAGCCAGGAGAAAGAT98520.1642378020674425No Hit
CCTCTCCCCTGGTGTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA97930.1632542423514479No Hit
CCTCTCCCCTGGTGCTGATCCATGGGTTTCCAGCCAGGAGAAAGATCGGA90580.1510014221606673No Hit
CCTCTCCCCTGGTGCTGATCCGGGTTTCCAGCCAGGAGAAAGATCGGAAG86750.14461661925853267No Hit
CCTTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA85060.14179930413983619No Hit
CCTCACCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA79990.13334735878374673No Hit
CCTCTCCCCTGGTGCTGATCGGGAAAAGGGAGGAGCTGAGGATTCAGAAT78970.1316469674103323No Hit
CCCCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA77480.1291630623648543No Hit
CCTCTCCCCTGGTGCTGATCACGGGTTTCCAGCCAGGAGAAAGATCGGAA71690.11951084074517818No Hit
CCTCTCCCCTGGTGCTGATCTCATGGGTTTCCAGCCAGGAGAAAGATCGG64690.10784148818253No Hit
CCTCTCCCCTGGTCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA62620.10439069392471834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)