FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003491597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5819575
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA88353915.182191139387328No Hit
CCTCTCCCCTGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA466560.8017080285072364No Hit
CCTCTCCCCTGGTGCTGATCATGGGTTTCCAGCCAGGAGAAAGATCGGAA465780.800367724447232No Hit
CCGCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA445530.765571369043272No Hit
CCTCTCCCCTGGTGCTGATCGTGACTGGGTTTCCAGCCAGGAGAAAGATC424480.7294003428085384No Hit
CCTCGCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA408750.7023708775984501No Hit
CTCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA339270.5829807159457521No Hit
CCTCTCCCCTGGTGCTGATCGGGTTTCCAGCCAGGAGAAAGATCGGAAGA282010.48458865123312267No Hit
CCTCTCCCCTGGTGCTGATCAGGGTTTCCAGCCAGGAGAAAGATCGGAAG267050.458882306697654No Hit
CCTCTCCCCTGGTGCTGACCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA236050.4056138120051722No Hit
CCTCTCCCCTGGTGCTGATCCCCATACCTATTTCACAGTGCTGCCCTCCG223050.38327541100509915No Hit
CCTCTCCCCTGGTGCTGATCGGAGGTCCTTGGGCAGCATCAGTCAAAGCA215780.37078308983044295No Hit
CCTCTCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA204260.3509878298673013No Hit
GCTCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA160040.27500289969628366No Hit
CCTCTCCCCTGGTGCTGATCCAGGGTTTCCAGCCAGGAGAAAGATCGGAA158770.2728206097524304No Hit
CCTCCCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA128130.2201707169337967No Hit
CCGCGCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA122910.21120098976299814No Hit
CCTCTCCCCTGGTGCTGATCATTCAGGGTTTCCAGCCAGGAGAAAGATCG105840.1818689509113638No Hit
CCTCTCCCCTGGTGCTGATCCAGTCACGGGTTTCCAGCCAGGAGAAAGAT102430.1760094164951908No Hit
CCTCTCCCCTGGTGCTGATCCATGGGTTTCCAGCCAGGAGAAAGATCGGA97100.16685067208516086No Hit
CCTCTCCCCTGGTGCTGATCACGGGTTTCCAGCCAGGAGAAAGATCGGAA95310.16377484610130463No Hit
CCCCTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA91710.1575888273628229No Hit
CCTCTCCCCTGGTGTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA90440.1554065374189696No Hit
CCTCACCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCA89700.1541349669005039No Hit
CCTCTCCCCTGGTGCTGATCCGGGTTTCCAGCCAGGAGAAAGATCGGAAG86000.14777711430817542No Hit
CCTTCCCCTGGTGCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA85020.14609314254047762No Hit
CCTCTCCCCTGGTGCTGATCGGGGTTTCCAGCCAGGAGAAAGATCGGAAG67830.11655490306422721No Hit
CCTCTCCCCTGGTGCTGATCATTTGAGGTCAGGAGTTCAAGACCAGCCTG66490.11425232942268121No Hit
CCTCTCCCCTGGTGCTGATCGGGAAAAGGGAGGAGCTGAGGATTCAGAAT64310.1105063514088228No Hit
CCTCTCCCCTGGTGCTGATCTGGGTTTCCAGCCAGGAGAAAGATCGGAAG64120.11017986708651406No Hit
CCTCTCCCCTGGTGCTGATCTCCAGCGGAGGGGACCCAACAGCGTCTGGA63350.10885674641189436No Hit
CCTCTCCCCTGGTCTGATCAGAGGTCCTTGGGCAGCATCAGTCAAAGCAA62850.10799757714266077No Hit
CCTCTCCCCTGGTGCTGATCACCAACACTTGTTATTTTCTGTTTATTTTT62580.10753362573727462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)