FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003491658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003491658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23401565
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC557028223.80303197670754TruSeq Adapter, Index 3 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGTCATCTCGTATGCC429690.18361592483237765TruSeq Adapter, Index 3 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATGCATCTCGTATGCC245240.10479640998369126TruSeq Adapter, Index 3 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGGCATCTCGTATGCC235090.10045909322731192TruSeq Adapter, Index 3 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)