FastQCFastQC Report
Wed 11 May 2022
EGAF00003492982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003492982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22049039
Sequences flagged as poor quality0
Sequence length35-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3856351.7489877903522233No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC538220.24410134155960264No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAAC535560.24289493977492624No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCC481070.21818184456928033No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGG412990.18730521543365222No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCC405770.18403069630381624No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTC389650.1767197200748749No Hit
GTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCG379750.17222972847025217No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT367070.16647891094029088No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC362530.16441986428524163No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC357930.16233360555986134No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCC355080.16104103221913663No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTG349370.15845135019263198No Hit
GTTACTCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATT348510.15806131051788697No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT348410.1580159570673352No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC344050.15603854662327915No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGG319180.14475914347106014No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG311780.14140298813023097No Hit
GTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA305890.1387316698927332No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA301380.13668622927284949No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATT290640.13181526868359206No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG284440.12900335474938385No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCT261530.11861287922797904No Hit
GTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCC258180.11709353863449559No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA250390.11356050483651463No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC245980.11156041766718268No Hit
GTTCAACTGCTGTTCACATGGAAGCCTTCTCCACTTCGGCCTTCAAAGTTC243660.1105082176143822No Hit
GTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGAC241190.10938798738575409No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT239430.10858976665604338No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC234550.10637651826911822No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTC233150.10574156996139379No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT231280.10489346043607616No Hit
GGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTAT228980.10385033107338601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)