FastQCFastQC Report
Wed 11 May 2022
EGAF00003493087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003493087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36975837
Sequences flagged as poor quality0
Sequence length35-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC3160770.8548204060938499No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC1642830.4442982588872836No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCC1600030.43272313213626507No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG1554220.4203339602562614No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGT1465010.3962073934932156No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1440170.38948949282743756No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACG1319280.3567951686935444No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1022770.27660496231633647No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1013880.2742006894935198No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC990260.2678127340295231No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT975720.2638804362968173No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTT954380.2581090997345104No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAC945640.25574539394469964No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT929100.25127220243858167No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC855280.23130781326194186No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA838510.22677241897188152No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATT818590.22138511698869723No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC818170.2212715292962807No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA796930.21552723742264443No Hit
GTTAGTTTCTTTTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTCGCC794870.21497011683602996No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG776580.2100236432781765No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG772920.2090338076728324No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC764070.20664035272548392No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG763960.20661060356794628No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG747250.20209143609108837No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCC712340.1926501352761805No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG712020.19256359227243458No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC707410.19131683212471973No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTG686440.18564556091049406No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGC684290.18506410010407606No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG682380.18454754655046754No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC674250.18234881336154746No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCC668940.18091274039313834No Hit
CTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTC662870.1792711277908327No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACT657320.17777014756961418No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGC652770.1765396142351017No Hit
GGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT645400.17454642068007817No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTC641550.17350520016625992No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC617850.16709560895132677No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC606720.16408553510228854No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG591220.15989360835834493No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC582260.15747040425345882No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA575120.1555394134823777No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCC570100.1541817701111134No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT548390.14831036820072524No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCG534140.14445650006516417No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT532340.14396969566909332No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACT525250.14205222724234748No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT517930.14007255603165927No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA517380.13992381024397094No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC514190.1390610846753787No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG513590.13889881654335506No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG513390.13884472716601384No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCC511240.13826326635959585No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC508630.1375573999852931No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG505990.13684342020438917No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG490470.13264608452271143No Hit
ACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTC483030.13063395968561847No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCT480380.12991727543584747No Hit
CGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCT480060.12983073243210155No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC471310.12746432217342368No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCAC467120.1263311497181254No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA465400.125865981072991No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGC451400.12207972465910642No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT450940.12195531909122165No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG444520.12021905007856888No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC444240.12014332495029119No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC433310.11718734047859417No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGC429420.11613530208930767No Hit
CTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGC421770.11406638340600646No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCT415740.11243558867916904No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATT414110.11199476025383821No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT410630.11105360508810119No Hit
CCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCT407770.1102801269921219No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGT405810.10975005109417808No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT401760.1086547412030186No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG395490.10695903922337173No Hit
GTTAGTTTCTTCTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTCGCC390730.105671712042651No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT389790.1054174919691473No Hit
CTGGTTAGTTTCTTTTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTC386550.10454124405621976No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG385550.10427079716951371No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGT382850.10354059057540738No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC382810.10352977269993915No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT375450.10153928361378269No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCT374810.10136619760629084No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCT371960.10059542397917862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)