FastQCFastQC Report
Mon 6 May 2024
EGAF00003593485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20821325
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG460390.22111465048453927Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG458740.22032219371245584Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGATGTATCTCGTA310290.14902509806652553Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTA224050.10760602411229833Illumina Paired End Sequencing Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)