FastQCFastQC Report
Sun 5 May 2024
EGAF00003593493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17695887
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG840180.4747882940256117Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTA373880.21128073433109062Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCAATATCTCGTA295980.16725920548656306Illumina Paired End Sequencing Primer 2 (100% over 36bp)
AGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG216320.12224309524580487Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)