FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003593493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17695887
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTATGCCG317480.17940892140642625Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA250370.1414848546444719Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA233930.13219456023877185Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCAATATCTCGTATGCCG230010.12997935621989448Illumina Paired End PCR Primer 2 (97% over 37bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTATGCCG182270.10300133584713782Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)