FastQCFastQC Report
Mon 6 May 2024
EGAF00003593496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19488621
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTA3678251.8873834120946782Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2273451.1665525231364497Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG285420.14645469271530295Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTA261830.13435019337694545Illumina Paired End PCR Primer 2 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)