FastQCFastQC Report
Fri 10 May 2024
EGAF00003593497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16418923
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTA828230.504436253218314Illumina Paired End PCR Primer 2 (97% over 37bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG222970.13580062468165544Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)