FastQCFastQC Report
Sat 1 Apr 2023
EGAF00003593504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24017015
Sequences flagged as poor quality0
Sequence length75
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA242300.1008868087895186Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)