FastQCFastQC Report
Mon 6 May 2024
EGAF00003593511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20901570
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTA3670861.7562604148874943Illumina Paired End PCR Primer 2 (97% over 38bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2373351.1354888651905097Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTA341370.16332265949399974Illumina Paired End Sequencing Primer 2 (100% over 36bp)
AGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG260910.12482794354682447Illumina Paired End PCR Primer 2 (100% over 31bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC256080.12251711235089038Illumina Paired End PCR Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)