FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003593511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20901570
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2873861.374949345910379Illumina Paired End PCR Primer 2 (97% over 38bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTATGCCG1897250.9077069330198642Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA216240.10345634323163284Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)