FastQCFastQC Report
Wed 1 May 2024
EGAF00003593512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23623926
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAGGCATCTCGTA3448231.4596346094209744Illumina Paired End PCR Primer 2 (97% over 38bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2556471.08215289871802Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGATCATCTCGTA496130.210011663599014Illumina Paired End Sequencing Primer 2 (100% over 36bp)
AGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG367920.15574041334196526Illumina Paired End PCR Primer 2 (100% over 31bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC278370.11783392819635483Illumina Paired End PCR Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)