FastQCFastQC Report
Fri 3 May 2024
EGAF00003593513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21179129
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTA3725851.7592083224952264Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2861071.350891247699563Illumina Paired End PCR Primer 2 (100% over 31bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC321990.1520317478589417Illumina Paired End PCR Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATTCATCTCGTA288330.13613874300496492Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGACCGTAGCTTATCTCGTATGCCGTCTTCT286100.13508581962931526TruSeq Adapter, Index 10 (96% over 26bp)
AGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG230170.10867774590730336Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)