FastQCFastQC Report
Wed 1 May 2024
EGAF00003593514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003593514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21880273
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCGCGATCTCGTA4662912.131102294747419Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG4505212.0590282397299156Illumina Paired End PCR Primer 2 (100% over 31bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACC543050.24819160163129592Illumina Paired End PCR Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGACCGGCCGCGATCTCGTATGCCGTCTTCT220850.10093566931271836Illumina Paired End Sequencing Primer 2 (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)